Silver Nanoparticles as an Antimicrobial Agent in theTissue Culture of Aglaonema commutatum Plant
DOI:
https://doi.org/10.56286/NTU-JAVS.2026.6.3.290-295Keywords:
Aglaonema commutatum, Benzyladenine, Contamination control, Gibberellic acid, Silver nanoparticles.Abstract
Aglaonema commutatum is a popular ornamental foliage plant valued for its attractive variegated leaves, shade tolerance, and adaptability to interior landscaping. Due to its commercial demand and the limitations of conventional propagation methods, micropropagation has become an essential technique for producing uniform and disease-free planting material. However, microbial contamination remains a critical challenge in in vitro culture, often reducing culture efficiency and increasing production costs. This study investigated the interaction between plant growth regulator (PGR) treatments and silver nanoparticle (AgNP) exposure duration on contamination control, browning incidence, and regeneration of A. commutatum bud explants cultured in vitro. Explants were exposed to AgNPs for 30, 45, and 60 minutes and cultured under four PGR combinations: T1 (0.5 mg L⁻¹ BA + 0.5 mg L⁻¹ GA), T2 (1.0 mg L⁻¹ BA + 0.5 mg L⁻¹ GA), T3 (1.5 mg L⁻¹ BA + 0.5 mg L⁻¹ GA), and T4 (2.0 mg L⁻¹ BA + 0.5 mg L⁻¹ GA). At 30 minutes, all treatments exhibited complete suppression of contamination and browning (0.00%), with T1 and T2 achieving 100% regeneration. Longer exposure durations (45–60 minutes) led to increased contamination (33.33 –100%), browning in some treatments, and a sharp decline in regeneration. These results indicate that short-term AgNP exposure combined with low BA concentrations is highly effective for disinfection and shoot regeneration. The use of AgNPs offers a promising, alternative to conventional sterilization methods in the micropropagation of ornamental plants.
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