Molecular investigation of the adhesion and biofilm genes FimH and CsgA, which are the main contributors to endometritis and metritis in cows with Escherichia coli infections
DOI:
https://doi.org/10.56727/NTU-JAVS.2026.6.2.189-194Keywords:
Genital tract infection, E.coli, PCR, FimH, CsgA.Abstract
Microorganisms infections of the genital tract are among the most serious problems impacting cattle herds, leading to financial losses in addition to infertility and decreased productivity. The study was conducted between September 2024 and February 2025 in various areas of Nineveh Governorate, including Nimrud and Shamsiyat districts and the veterinary hospital in Bab Sinjar, To isolate Escherichia coli from genital tract infections in cows and molecular detection of virulence genes FimH and CsgA. In our study, E. coli had the highest isolation rate (44%). E. coli has shown absolute resistance to the antibiotics Imipenem, Tetracycline, and Cefixime .The UidA gene was used to confirm the molecular diagnosis of E. coli. All 23 bacteria isolates selected for the study were positive for the FimH gene, which regulates adhesion, while isolates 20 and 22 were negative for the CsgA gene.
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