Evaluation of the genotoxicity and genomic instability in patients with HBV, HCV and HCC using micronucleus and comet assay

Abstract

On somatic cells, the hepatitis B virus (HBV) and the hepatitis C virus (HCV) are mutagenic. HBV and HCV may be causing these mutagenesis effects via integrating into host DNA or by viral proteins. The purpose of this research was to investigate if HBV and HCV had a genotoxic effect on renal epithelial cells' DNA. A total of 145 samples were taken from participants between the periods of 5 \1 2020 and 15\9 2021. (40 healthy controls, HBV 38, 44 HCV, and HCC 23) were used to perform cytogenetic analysis in renal epithelial cells using the micronucleus (MNi) test and comet assay. For the comet experiment, 100 cells were examined for each participant. A total of 100 cells were examined, with MNi scores assigned to each participant. The frequency of MNi was found to be considerably greater in the HBV, HCV, and HCC patient groups than in the control group. There was no significant difference in MNi scores between the HBV, HCV, and HCC patient groups, however there was a significant difference between the study groups and healthy carriers. In conclusion:  due to their levels of DNA damage and MNi, chronic HBV, HCV, and HCC patients are afflicted by genomic instability much like other patients.